New method for monitoring programmed cell death and differentiation in submerged Streptomyces cultures.
نویسندگان
چکیده
Vital stains were used in combination with fluorimetry for the elaboration of a new method to quantify Streptomyces programmed cell death, one of the key events in Streptomyces differentiation. The experimental approach described opens the possibility of designing online protocols for automatic monitoring of industrial fermentations.
منابع مشابه
Mycelium differentiation and antibiotic production in submerged cultures of Streptomyces coelicolor.
Despite the fact that most industrial processes for secondary metabolite production are performed with submerged cultures, a reliable developmental model for Streptomyces under these culture conditions is lacking. With the exception of a few species which sporulate under these conditions, it is assumed that no morphological differentiation processes take place. In this work, we describe new dev...
متن کاملStreptomyces developmental cycle and secondary metabolite production
Streptomyces is an extremely important bacterium for industry, since approximately two thirds of all antibiotics are synthesized by members of this genus. Furthermore, Streptomycetes produce large numbers of eukaryotic cell differentiation and apoptosis inducers. Streptomyces is a mycelial soil bacterium characterized by a complex developmental cycle that includes programmed cell death (PCD) ph...
متن کاملDifferentiation and anaerobiosis in standing liquid cultures of Streptomyces coelicolor.
Streptomyces coelicolor differentiates on solid agar media by forming aerial hyphae that septate into spores. We here show that differentiation also occurs in standing liquid minimal media. After a period of submerged growth, hyphae migrate to the air interface, where they become fixed by a rigid reflecting film. Colonies that result from these hyphae form sporulating aerial hyphae. In addition...
متن کاملNew Insights in Streptomyces Fermentations.
Copyright: © 2012 Sanchez J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Streptomyces is a very important industrial bacterium, which produces two thirds of clinically relevant secondary metabolites...
متن کاملImpact of Duration and Severity of Persistent Pain on Programmed Cell Death
Programmed cell death is a highly regulated form of cell death, mostly distinguished by the activation of a family of cystein-aspartate proteases (caspases) that cleave various proteins resulting in morphological and biochemical changes characteristic of this form of cell death. Several recent studies have addressed the role of programmed cell death in inflammatory and chronic pain states. Casp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 76 10 شماره
صفحات -
تاریخ انتشار 2010